Globalization process Essay Example for Free

Globalization process EssayDue to the emergence of globalization process, many changes are taking place within the mainstream business activity. As the organizations becoming to more and more globalized, the need to have a uniform set of international standards is potently felt.This argument is based on the concomitant that since organizations are becoming global and have to face the different legal as well as cultural environments therefore in a bid to reduce the bottlenecks for the international firms as well as creating a uniform set of accounting rules and regulations, it is critical that a convergence shall be made to the international accounting standards so that a uniformity can be achieved. The experience of EU and Canada has been successful so as those of other countries who are making a transition to the international accounting standards. This experience is also considered as a strong signal for the US to adapt to the new standards because it is connected with the global world.The complexities of the international trade have led much international business to remove the conflict between the different standards in place and as such the effort from the US is one of the attempts to converge to the International standards to achieve the desired results. However, it is also critical to note that many plead that such transition will be costly for the American firms because an earlier transition to Sarbanes Oxley has been a costly proposition for the many companies and most of them may be reluctant to adapt to the new standards.The costs, however, may be relatively high but the added benefits which US firms may enjoy after this convergence may greatly outweigh the benefits. The uniformity of the standards will allow international firms, especially to be more flexible and support the initiatives taken by the Security and Exchange kick to make a phased transition to the adaptation of the IFRS.The process of this convergence can be difficult to asse ss as it is a phased process however, given the fact that more than 85 countries have adapted the IFRS itself indicate that more and more countries are now willing to adapt to the international standards in order to aid the businesses to adapt a uniform set of standards. Such large scale adaptation of the standards however, also creates cultural as well language problems as the settle and accurate interpretation of the standards itself is an issue to deal with.Language and culture therefore are two of the potential impediments which can restrict the true applicability of the standards level off if the standards are fully implemented and adapted by the countries. If we assess the globalization of accounting standards with special reference to US, we will also come to know that a couple of(prenominal) of the international firms in US have already adopted the standards for their foreign subsidiaries and as such this process may not be as modify to them as they may perceive it.Howe ver, what is also critical to note that those firms which have only the domestic presence within the US market may pay off it difficult to cope with the increasing costs as well as adapting to the new standards? This will not only require investment into improving the readiness level of the employees as well as would require additional funds to implement the new system.

Philosophy of Nursing: Reflection

Philosophy of care for ReflectionA philosophy is a statement of belief astir(predicate) something. Humans, their wellness, and the environment in which they live and function are phenomena of particular interest to nursing. Learning to reflect critically on iodines radiation pattern requires considerable effort. It is besides ingrained to being an excellent practitioner. Being able to think about and to explain your beliefs about the basic concepts of your practice provides the framework for your practice. Do remember that what you write today is not written in stone. As you learn new information and expand your professional practice your thoughts and beliefs about these phenomena may very well change. View this assignment as an opportunity to articulate your thoughts about these four concepts. My Belief is Good anguish check up on and community health services financial aid rebuild a someone after functioning You need to include the following items 1. An introductio n to the paper that includes a brief overview of the paradigm.2. A statement and explanation of your beliefs in relation to each(prenominal) of the four concepts included in the paradigm of nursing ( sympathetics, health, nursing, environment). While there is no minimum unavoidableness for the length of each statement and explanation, many fall into the range of three (3) to five (5) paragraphs for each component of the paradigm. Some questions you may want to ask and response for your self are What is the essence of human? What is health? Illness? Wellness? How are they related? What is the reason for nursings existence? How do I facilitate a clients purport toward health? These are just suggestions. You are NOT required to respond to the questions listed in this paragraph. As you consider your explanation of how you have reached this belief, i.e. This I believe about . . . because. . ., you may want to review some of the information from previous NRSE 300 modules. The Paradig m Online Writing Assistant has guidelines about writing a Thesis/ plunk for Essay. This can help you in the construction of the paper. You need to document your explanation with material from the textbook, from the videos, from your practice, and from at least dickens sources that are not listed in your secernate materials. 3. Choose one of the soak up theorists you viewed. Compare your beliefs to those expressed or implied by the jean Watson. How similar are your beliefs? What differences exist? 4. The final section of the paper is to be a statement of your philosophy of nursing. It may help to go to the Ohio School of Nursing website and read the Philosophy of the School of Nursing. It will bemuse you a virtuoso of one style of writing a philosophy. The philosophy is a distillation of the thinking you did to write your beliefs and explanations. It usually takes the form of one to three sentences about each of the components with linking comments about how they relate to eac h other to form your personal philosophy of nursing. 5. Paper will be typed. Double-space the body of the paper. Use APA format for headings and references. shamble sure that your name is on each page of the paper. Use the running head style as explained in the APA Manual. Proofread the paper. Do NOT deposit solely on spell check. It will not tell you when you wrote of when you intended to write or. There is no specified length for this paper. They usually run mingled with 3 and 6 pages of double-spaceIntroductionNursing refers to a contact or a bond that exists between two or more persons in which one of the two persons provide professional care to the other in addition to providing advice with an aim of improving the health of the other private an preventing illness. Philosophy of nursing may refer to beliefs that distinguishable individuals have towards nursing.Humans refer to the primates in the family of hominids. They have a brain which is highly expanded enabling them to put to work problems through and through reasoning, get word of emotions, use of language and introspection. Humans are always craving to understand the world around them and influence the natural occurrences. Health refers to a state of well being where there is no sickness and reflects the social, physical, spiritual and mental fitness of an individual. Good health may be brought about by a balanced diet, exercise and rest. Nursing refers to a scholarship in which the person involved aims at ensuring a good health for individuals, families and communities. In general, the science of nursing aims at upholding the quality of life. purlieu is delimit as the extensive information on issues, programs and policies affecting our surrounding. It refers to anything internal or external that may stimulate some impact in the life of an individual including all the living and non living organisms that exist on the earth.HumansA good pain control after surgery when accompanied by appr opriate community health services enables an individual to rebuild him or herself. It is however the responsibility of every person to ensure that the nurse or the doctor knows about him or herself in the lead the pain control is administered on him or her. This the patient role could do by enabling the doctor to know about his or her drug addiction either the past or present.Surgery of the stomach is a very painful one and the drug history of a person may affect the persons tolerance to the pain or response to the pain relievers. The pain control in patients also depends on the age of the patient as well the conditions of health of the individual before the surgery. Controlling pain after surgery is a very essential step in the life of a patient as it helps the individual to gain the best results on functionality. This is appropriate because the pain after surgery increases the functionality of the patient.Patients who before the surgery were on pain medication do require an admin istration of extended pain medication. Otherwise the patient may require shorter acting pain medications for the control of pain.NursingThe belief that good pain control and community health services help rebuild a person after surgery relates to nursing in the sense that nurses have been found to be mediums through which terminal care at home can be improved. Pain control is one of the components of quality terminal care in the community. Patients who have undergone surgery should be given enough advice by the nurses on where to get help if necessary. In addition to this they should be given appropriate analgesics to carry home which should be accompanied by sufficient instructions on their usage. Nevertheless, patients should be given both(prenominal) verbal and written information about pain so that and how pain can be reduced. The nursing staff should always be there for patients to advice them and to cover with them their choice of a pain relieving techniques.The pain that is experienced by patients after surgery is usually very great that it should not be underestimated. This is because the pain does different implications on both the patients and the community health services. A proper pain control after surgery therefore requires the intervention of the nurse which will give way for community health services thus helping the patient to gain his or her health back. Nurses facilitates the process of pain control and ensures that it well managed to speed the process of recovering of a patient.HealthThe health of a person enables that person to function mentally, spiritually, physically and socially in full ability. The belief that a good pain control and community health service helps an individual to rebuild after a surgery relates to health in the sense that an individual will only have a good health when he or she is not in pain.A person is considered to fully healthy if he or she is not feeling any pain. Surgery involves a lot of pain therefore aft er a surgery an individual requires pain remainder which is achieved through proper pain control by the nurses to enable him or her to function properly both physically, mentally, spiritually and socially.When a person is in pain, he or she will not perform any physical activity in addition the person will not be able to socialise freely with other people. The person will also not think properly thus lacking both mental and spiritual thinking. This is because pain makes a person un at ease creating a feeling that something is wrong in the body. The pain in the body i.e. physical pain sends a signal to the brain through the spinal cords that something is wrong thus preventiveping the mind from working properly. It is therefore essential that after surgery a patient should receive a good pain control and community health service to enable him or her to rebuild his health. Effective pain focal point is therefore considered by nurses as part of recovery because it when it is well co ntrolled it speeds up the process of ameliorate and reduce complications that a person may experience as a result of surgery.EnvironmentThe surroundings in which individuals live do affect the activities performed in that particular environment. Nurses do require an enabling environment to enable them to perform their duties properly. After surgery it will for the well being of the patient to have a conducive environment in which physical, mental, spiritual and social healing is possible.The nature of the environment in which pain control is conducted is very essential and should be set to fit the requirements of different patients. Pain control will enable an individual to feel comfortable as he or she heals, get well faster and avoid some problems associated with surgery.The nurse theorist viewed was Margaret Newman who also came up with a nursing opening of health as an expanding consciousness which requires every person to find the meaning of life and find connections with oth er people in the rest of the world. According to Margaret, health means increasing awareness. She put forward that the role of a nurse is to identify the modus vivendi of an individual so that the nurse can work inwardly that lifestyle to help the individual achieve the goals of his or her life. Newmans theory includes the health of every individual irrespective of the condition of illness i.e. whether sick or well.Jean on the other hand developed a caring theory which involves the caring actions taken by nurses in their interaction with the patients. The caring theory involves the mystical respect offered to patients by the nurses for the mysteries of life and the ability of the patients to change their lifestyles. Watsons caring theory also requires a nurse to help the patients to acquire self control, knowledge and healing not regarding their health conditions.The difference that exists between my beliefs on Newmans theory and Watsons caring theory is that Newmans theory was fo cused on helping every person whether in the absence or presence of sickness to understand his or her status. It focused on the adjustments that a nurse should put to ensure that a person meets his or her requirements. Watson on the other hand focused on caring for the patients, respecting them and helping them to understand themselves by acquiring self control, knowledge and healing.The similarity between the two theories is that both the two believe that it is the role of a nurse to ensure appropriate lifestyles for different persons whether sick or not. They both believe that a nurse should be able to identify or recognize and accurately detect the health status of a person and be able to help that person accordingly. They believe that a nurse must come together and engage in an interaction so that the nurse can understand the unique lifestyle of the person and provide care in a manner that fits that lifestyle. Finally both the beliefs involve human and nursing in the science of nursing in order to provide desired care to persons.The statement of my philosophy of nursing is the belief that Good pain control and community health services help rebuild a person after surgery.ConclusionSurgery is a very painful exercise which usually requires an immediate pain control to stop the patient from suffering. Pain management especially after surgery is therefore very essential for the well being of the patient and nurses should ensure that it is provided to enable the patient to rebuild his or her health.ReferenceNewman, M. A. (1972). Nursings theoretical evolution. Nursing Outlook, 20(5), 449-453.Newman, M.A. (1979). Theory development in nursing. Philadelphia F.A. Davis.Newman, M. A. (1982). Time as an index of expanding consciousness with age. Nursing Research, 31(5), 290-293.Newman, M.A. (1983). Newmans health theory. In I. Clements F. Roberts (Eds.), Family health A theoretical approach to nursing care (pp. 161-175). New York John Wiley.Engle, V. F. (1984). New mans conceptual framework and the measurement of older adults health. Advances in Nursing Science, 7(1), 24-36.Newman, M. A. (1984). Nursing diagnosis Looking at the whole. American Journal of Nursing, 84(12), 1496-1499.Newman, M.A. (1986). Health as Expanding cognizance. St. Louis Mosby.Newman, M. A. (1987). Aging as increasing complexity. Journal of Gerontological Nursing, 13(9), 16-18.Newman, M.A. (1990). Newmans theory of health as praxis. Nursing Science Quarterly, 3(1), 37-41Gustafson, W. (1990). Application of Newmans theory of health Pattern recognition as nursing practice. In M. E. Parker (Ed.), Nursing theories in practice (pp. 141-161). New York depicted object League for Nursing.Kalb, K. A. (1990). The gift Applying Newmans theory of health in nursing practice. In M. Parker (Ed.), Nursing theories in practice (pp. 163-186). New York national League for Nursing.Newman, M. A. (1990). Shifting to high consciousness. In M. E. Parker (Ed.), Nursing theories in practice (p p. 129-139). New York National League for Nursing.Newman, M. A. (1990). Toward an integrative model of professional practice. Journal of Professional Nursing, 6(3), 167-173.Newman, M. A., Lamb, G. S., Michaels, C. (1991). Nurse case management The coming together of theory and practice. Nursing Health Care, 12(8), 404-408.Newman, M. A., Sime, A. M., Corcoran-Perry, S. A. (1991). The focus of the discipline of nursing. Advances in Nursing Science, 14(1), 1-6.Newman, M. A. (1992). Prevailing paradigms in nursing. Nursing Outlook, 40(1), 10-14.Marchione, J. (1993). Margaret Newman Health as expanding consciousness. Newbury Park, CA Sage.Newman, M. A. (1994). Health expanding consciousness (2nd ed.). New York National League for Nursing.Newman, M.A. (1994). Theory for nursing practice. Nursing Science Quarterly, 7(4), 153-157.Newman, M. A. (1995). A developing discipline Selected works of Margaret Newman. New York National League for Nursing.Marchione, J. (1995). Margaret Newman Heal th as expanding consciousness. In C. M. McQuiston A. A. Webb ( Eds.), Foundations of nursing theory Contributions of 12 key theorists (pp. 261-316). Thousand Oaks, CA Sage.Engle, V. F. (1996). Newmans theory of health. In J. J. Fitzpatrick A. L. Whall (Eds.), Conceptual models of nursing depth psychology and application (3rd ed., pp. 275-288). Stamford, CT Appleton Lange.Newman, M.A. (1996). Theory of the nurse-client partnership. In E. Cohen (Ed.), Nurse case management in the 21st Century (pp. 119-123). St. Louis Mosby Year-book.Newman, M. A. (1997). Experiencing the whole. Advances in Nursing Science, 20(1), 34-39.Newman, M. A. (1997). Evolution of the theory of health as expanding consciousness. Nursing Science Quarterly, 10(1), 22-25.Yamashita, M., Jensen, E., Tall, F. (1998). Therapeutic touch Applying Newmans theoretic approach. Nursing Science Quarterly, 11(2) 49-50.Desai, S., Keffer, M. J., Hensley, D. Kilgore-Keever, K. A., Langfitt, J. V. Peterson, L. (1998). Margare t A. Newman Model of Health. In A. M. Tomey M. R. Alligood (Eds.), Nursing theorists and their work (4th ed., pp. 496-515). St. Louis Mosby.Newman, M. A. (1999). The rhythm of relating in a paradigm of wholeness. Image Journal of Nursing Scholarship, 31(3), 227-230.Pharris, M. D. (2001). Margaret A. Newman Health as expanding consciousness. In Parker, M. (Ed.), Nursing theories and nursing practice (pp. 263-274). Philadelphia Davis.Newman, M. A. (2002). Caring in the human health experience. world(prenominal) Journal for Human Caring. 6(2), 8-12.Newman, M. A. (2002). The pattern that connects. Advances in Nursing Science, 24(3), 1-7.Witucki, J. M. (2002). Newmans theory of health as expanding consciousness in nursing practice. In M. R. Alligood A. M. Tomey (Eds.), Nursing theory use application (2nd ed., pp. 429-449). St. Louis Mosby.Newman, M. A. (2003). A world of no boundaries. Advances in Nursing Science, 26(4), 240-245.Endo, E. (2004). Nursing praxis with Margaret Newmans theory of health expanding consciousness. Nursing Science Quarterly, 17(2), 110-115.Health as Expanding Consciousness A website by Margaret Newman.ASSUMPTIONS deal with human life, nursing science, and the process of nursing.Watsons conception of human life is tied to notions that ones soul possesses a body that is not hold by objective space and time. The lived world of the experiencing person is not distinguished by external and internal notions of time and space, but shapes its own time and space, which is free by linearity.Nursing is a human science of persons and human health-illness experiences that are mediated by professional, personal, scientific, esthetic, and ethical human care transactions.The process of nursing is human care.THE THEORY OF HUMAN CARINGThe main concept of the theory is TRANSPERSONAL HUMAN CARING, which is best understood inwardly the concepts of three ancillary concepts LIFE, ILLNESS, and HEALTH.HUMAN LIFE is defined as spiritual-mental-physical being- in-the-world, which is continuous in time and space.ILLNESS is not necessarily disease. Illness is prejudiced turmoil or dis consistency with a persons inner self or soul at some level or disharmony within the spheres of the person, either consciously or unconsciously.HEALTH refers to unity and harmony within the mind, body, and soul.TRANSPERSONAL HUMAN CARING and caring transactions are those scientific, professional, ethical, yet esthetic, creative and personalized giving-receiving behaviors and responses between nurse and patient that allow for contact between the subjective world of the experiencing persons through physical, mental, or spiritual routes or some combination thereof.THE GOAL OF NURSING is to help persons gain a higher degree of harmony within the mind, body, and soul which generates self-knowledge, self-reverence, self-healing, and self-care processes while increasing diversity.Nursing interventions or CARATIVE FACTORS areHumanistic-altruistic system of valuesFait h-hopeSensitivity to self and othersHelping-trusting, human care relationshipExpressing dogmatic and negative feelingsCreative problem-solving caring processTranspersonal teaching-learningSupportive, protective, and/or corrective mental, physical, societal, and spiritual environmentHuman needs assistanceExistential-phenomenological-spiritual forcesReturn to TopThis page was brave modified on 6/1/02

Osmotic Pressure Effect on Plasma Membrane of Blood Cells

Osmotic Pressure Effect on Plasma Membrane of Blood mobile phonesSyed IbrahimIntroductionThe plasma tissue layer is vital as it defines the boundary in the midst of cellphones and their environment (Heidcamp et al., 2014). Plasma membranes are crucial in maintaining electrochemical gradients, controlling material exchange, and allowing signal transduction (Zhao et al., 2004). The purpose of this lab was to identify isoosmotic solutions by examining the rear of osmotic pressure on the plasma membrane of line of reasoning cells and Elodea base hit cells as well as to understand cell viability and membrane integrity development the Trypan ghastly exclusion balk.To examine the effects of osmosis, tool business cells and go for cells from Elodea leaves were examined in this lab. Plasma membranes are found in both animal cells and plant cells however, cell walls are only present in plant cells (Freeman et al., 2011). It was evaluate that these cells would shrink in hypertonic solutions, stay the same size in isotonic solutions, and swell (in the case of Elodea guard cells) or even burst (in the case of animal blood cells) in hypotonic solutions (Heidcamp et al., 2014). Since glucose has a avant-gardet Hoff factor of 1 while potassium chloride (KCl) has a vant Hoff factor of 2, it was predicted that 0.15M glucose and 0.07M KCl would be the isoosmotic solutions for both the animal blood and the Elodea guard cells. Concentrations greater than the isoosmotic solution were expected to be hyperosmotic, while assiduousnesss less than it were expected to be hypoosmotic.Viable cells produce intact plasma membranes (Heidcamp et al., 2014). In this lab macrophage cells, a type of leukocytes, were tempered with various compounds and the effect of these compounds on the membrane of the cells was examined using Trypan blue, which differentially stains viable and non-viable cells based on whether the cells have an intact plasma membrane (Freeman et al., 2011 Strobe r, 2011). Of the three any(prenominal) known compounds, it was predicted that hepes-buffered RPMI (HPMI) would impedeage the membrane the least as it contains vitamins and supplements, and it was predicted that methyl -cyclodextrin (MCD) would damage the cell the near as it removes cholesterol from plasma membranes (LifeTechnologies, 2013 Rodal et al., 1999).ResultsThe following three equations were used in calculations. Equation 1 was used to calculate osmolarity, in order to determine the osmotic effect of a particular solution, (Heidcamp et al., 2014). Equation 2 was used to calculate osmotic pressure and Equation 3 was used to convert Celsius temperature to kelvin temperature (Heidcamp et al., 2014 USMA, 2012).Where = osmolarity = vant Hoff factor = molar concentration Where = temperature = temperature Where = osmotic pressure (kPa) = vant Hoff factor = molar concentration = ideal gas constant = (Chieh, 2002) = temperature Part A Lab 2 Report Sheets recreate refer to atta ched sheets.Part B Answers to Assigned Questions base on the observations of my colleagues and myself, 0.15M glucose and 0.15M potassium chloride (KCl) were the isotonic solutions for the Eloda guard cells, while 0.15M glucose and 0.035M potassium chloride (KCl) were the isotonic solutions for the animal blood cells.Solutions with solute concentrations greater than the isotonic solution were hypertonic, while concentrations less than it were hypotonic. Based on the observations from the lab, 0.6M glucose and 0.3M glucose were hypertonic for both the plant and blood cells, while 0.07M glucose and 0.035M glucose were hypotonic for the plant and blood cells. Additionally, 0.6M KCl, and 0.3M KCl, were hypertonic for the plant cell, while 0.07M KCl and 0.035M KCl were hypotonic for the plant cell. As well, 0.6M KCl, 0.3M KCl, 0.15M KCl, and 0.07M KCl were hypertonic for blood cells while there were no observed hypotonic KCl solutions for blood cells.It is expected that the isotonic solut ions of glucose and KCl will have the same osmolarity. Based on Equation 1, osmolarity is the product of the vant Hoff factor and molar concentration. Since glucose has a vant Hoff factor of 1 while potassium chloride (KCl) has a vant Hoff factor of 2, it is expected that glucose will have double the molar concentration of KCl to control the same osmolarity in the isotonic solutions. The observations from this lab reveal that for plant cells, glucose and KCl had the same concentrations for isotonic solutions while for animal cells, glucose had approximately four times the molar concentration of KCl for isotonic solutions. This may have occurred overdue to the subjective nature of classifying tonicity, and since these observations were split up amongst four groups, their subjective impressions may have been different. Ideally, one group to do all four sets, but due to limited time in the lab, this was not possible.Tween-20 is a non-ionic type of surfactant that send word be used s olubilizing means for membrane proteins (Iwahashi et al., 1991 Sigma, 2003). bovid serum albumin (BSA) is a protein that adsorbs onto negatively charged portions of the membrane bilayer, forming temporary gaps in the membrane, thus increasing the permeability of the membrane (Tsunoda et al., 2001). Based on this information, Tween-20 would apt(predicate) cause more damage on the membrane bilayer, resulting in more blue cells (after the Trypan blue exclusion test). Therefore, the odd unknown is most likely Tween-20, and the even unknown is most likely BSA. look to Engine Web of accomplishmentSearch hurt Tween 20 sorted by relevanceReference Iwahashi, K., Tsubaki, M., Miyatake, A., Miura, S., Hosokawa, K., Ichikawa, Y. (1991). catalytic properties of cytochrome P-450scc from bovid and porcine adrenocortical mitochondria Effect of tween20 concentration. The Journal of Steroid Biochemistry and Molecular Biology, 38(6), 727-731.Search Engine GoogleSearch Terms Tween-20Reference Sigm a. (2003). Tween-20 (P5927) Product Information. SigmaAldrich. Retrieved October 6, 2014, from https//www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/p5927pis.pdfSearch Engine Web of ScienceSearch Terms bovine serum albumin sorted by relevanceReference Tsunoda, T., Imura, T., Kadota, M., Yamazaki, T., Yamauchi, H., Kwon, K. O., et al. (2001). Effects of lysozyme and bovine serum albumin on membrane characteristics of dipalmitoylphosphatidylglycerol liposomes. Colloids and Surfaces B Biointerfaces, 20(2), 155-163.Based on the Trypan blue exclusion assay observations recorded in Table 2.2, unknown 1 (most likely Tween-20) caused the most damage to the plasma membrane as it had the highest percentage of blue cells (60%). Tween-20 belongs to the class of polyoxyethylene sorbate combounds (Boxtel et al., 1990). Among many functions, these compounds solubilize the proteins and lipids found in the plasma membranes, thereby lysing the membranes (Boxtel et al., 1990). This allows Trypan blue to enter the lysed membranes, making them blue.Discussion and ConclusionsSummary of findingsIn the first part of this lab, the effects of osmosis were examined on animal blood cells and Elodea guard cells. It was determined that isoosmotic solutions for the Eloda guard cells were 0.15M glucose and 0.15M potassium chloride (KCl), while the isoosmotic solutions for the animal blood cells were 0.15M glucose and 0.035M KCl. Solutions with solute concentrations greater than the isotonic solution were hypertonic, while concentrations less than it were hypotonic. It was expected that the isotonic solutions of glucose and KCl would have the same osmolarity, since neither of them can easily diffuse across the membrane without the use of a channel or transport protein (Heidcamp et al., 2014) . Based on Equation 1, osmolarity is the product of the vant Hoff factor and molar concentration. Since glucose has a vant Hoff factor of 1 while potassium chloride (KCl) has a vant Hoff factor of 2, it was expected that glucose would have double the molar concentration of KCl to obtain the same osmolarity in the isotonic solutions. The results from this lab do not reflect this theoretical expectation. This may have occurred due to the subjective nature of classifying tonicity. Ideally, one group should to do all four sets of observations for Table 2.1, but due to limited time in the lab, this was not possible.In the second part of this lab, membrane integrity and permeability was examined using the Trypan blue exclusion. Normally Trypan blue is not permeable in living cells. However, if the plasma membrane is not intact, Trypan blue is able to enter the cell, staining it blue (Heidcamp et al., 2014 Strober, 2011). Once treated with the specific compounds (such as glycine or Tween-20), the Trypan blue exclusion assay was performed on the RAW macrophage cells. Afterwards, the number of blue (non-viable) and non-blue (viable) cells were counted a nd their respective percentages were calculated. Glycine is smallest of the twenty common amino acids, and nearly all (97%) of treated cell remained viable (Freeman et al., 2011). methyl group -cyclodextrin (MCD) is a compound that removes cholesterol from plasma membranes, making the membrane more permeable, and it resulted in only 55% viable cells (Rodal et al., 1999). Hepes-buffered RPMI (HPMI) is a medium that contains vitamins and supplements that can be used for cell growth, and nearly all (98%) of treated cells remained viable (LifeTechnologies, 2013). In addition to these three compounds, the cells were treated with two unknowns. The odd unknown resulted in relatively few (40%) viable cells, while the even unknown resulted in many (85%) viable cells. Tween-20 belongs to a class of detergents which are known to solubilize membrane proteins, thereby lysing the membranes (Boxtel et al., 1990 Iwahashi et al., 1991). Bovine serum albumin (BSA) is a protein that forms temporary gaps in the membrane, thus increasing the permeability of the membrane (Tsunoda et al., 2001). Based on the literature, Tween-20 would cause more damage on the plasma membrane resulting in fewer viable cells. Therefore, the odd unknown was determined to be most likely Tween-20, and the even unknown was most likely BSA.Answer to questionsAn organism that thrives in high-salt environments is known as a halophile, such as Halomonas meridian (James et al., 1990 Ventosa, 1998).Search Engine Web of ScienceSearch Terms halophileReference James, S., Dobson, S., Franzmann, P., Mcmeekin, T. (1990). Halomonas meridiana, a New Species of Extremely Halotolerant Bacteria Isolated from polar Saline Lakes. Systematic and Applied Microbiology, 13(3), 270-278.Search Engine Web of ScienceSearch Terms halophileReference Ventosa, A., Nieto, J., Oren, A. (1998). Biology of Moderately Halophilic aerophilous Bacteria. Microbiology and Molecular Biology Reviews, 62(2), 504-544.Methyl -cyclodextrin (MCD) is a compound that removes cholesterol from plasma membranes (Rodal et al., 1999). then it is expected, that MCD makes the plasma membrane more permeable, and allows Trypan blue to enter the cell. The results from this lab agree with this, as a relatively large percentage (45%) of cells treated with MCD was stained blue in the Trypan blue exclusion assay.Search Engine Web of ScienceSearch Terms methyl genus Beta cyclodextrinReference Rodal, S. K., Skretting, G., Garred, O., Vilhardt, F., Deurs, B. V., Sandvig, K. (1999). Extraction of Cholesterol with Methyl-beta -Cyclodextrin Perturbs Formation of Clathrin-coated Endocytic Vesicles. Molecular Biology of the Cell, 10(4), 961-974.The predictions are belowAcetone is an organic solvent that solubilizes lipids from the plasma membrane (Jamur Oliver, 2010). High concentrations of dimethyl ketone can disrupt lipid packing in the membrane, thereby increasing membrane fluidity and permeability (Posokhov Kyrychenko, 2013). Therefore is p redicted that acetone will increase cell permeability and allow some of the Trypan blue into the cell, staining some cells blue.Methanol is an organic solvent that dissolves lipids from the plasma membrane (Jamur et al., 2010). Methanol can increase the rotational mobility of membrane lipids, increasing its fluidity (Joo et al., 2012). Similar to acetone, it is expected that methanol will increase cell permeability and allow some of the Trypan blue into the cell, staining some cells blue.Saponin is a detergent that selectively removes cholesterol from the plasma membrane, resulting in small holes in the membrane (Jamur et al., 2010). This would act in a manner similar to MCD, likely causing the cell to be permeable to Trypan blue and staining many cells blue.Triton X-100 is a non-ionic detergent that non-selectively solubilizes proteins from the plasma membrane (Jamur et al., 2010). This would act in a manner similar to Tween-20, likely causing the cell to be highly permeable to Try pan blue and staining a large percentage of them blue.Search Engine PubMedSearch Terms cell membrane permeabilityReference Jamur, M. C., Oliver, C. (2010). Permeabilization of cell membranes. Immunocytochemical Methods and Protocols, 588, 63-68.Search Engine Web of ScienceSearch Terms effect of acetone on membranesReference Posokhov, Y. O., Kyrychenko, A. (2013). Effect of acetone accumulation on structure and dynamics of lipid membranes studied by molecular dynamics simulations. computational Biology and Chemistry, 46, 23-31.ReferencesAlberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2008).Molecular Biology of the Cell(5th ed.). New York Garland Science.Boxtel, R. M., Lambrecht, R. S., Collins, M. T. (1990). Effect of polyoxyethylene sorbate compounds (Tweens) on colonial morphology, growth, and ultrastructure of Mycobacterium paratuberculosis. Apmis, 98(7-12), 901-908.Chieh, C. (2002). The Ideal Gas Law. Ideal Gas. Retrieved October 5, 2014, from http//ww w.science.uwaterloo.ca/cchieh/cact/c120/idealgas.htmlFreeman, S., Harrington, M., Sharp, J. (2011). Biological Science (Canadian ed.). Toronto Pearson Canada.Heidcamp, W., Antonescu, C., Botelho, R., Victorio-Walz, L. (2014).Laboratory Manual Cell Biology BLG311(Fall 2014 ed.). Toronto Ryerson University.Iwahashi, K., Tsubaki, M., Miyatake, A., Miura, S., Hosokawa, K., Ichikawa, Y. (1991). Catalytic properties of cytochrome P-450scc from bovine and porcine adrenocortical mitochondria Effect of tween20 concentration. The Journal of Steroid Biochemistry and Molecular Biology, 38(6), 727-731.James, S., Dobson, S., Franzmann, P., Mcmeekin, T. (1990). Halomonas meridiana, a New Species of Extremely Halotolerant Bacteria Isolated from Antarctic Saline Lakes. Systematic and Applied Microbiology, 13(3), 270-278.Jamur, M. C., Oliver, C. (2010). Permeabilization of cell membranes. Immunocytochemical Methods and Protocols, 588, 63-68.Joo, H., Jang, H., Yun, I., Bae, S., Chung, I., Bae, M ., et al. (2012). The Effect of Methanol on the Structural Parameters of Neuronal Membrane Lipid Bilayers. The Korean Journal of Physiology Pharmacology, 16(4), 255.LifeTechnologies. (2013). RPMI 1640 Medium, HEPES. Cell elaboration Transfection Reagents. Retrieved October 6, 2014, from http//www.lifetechnologies.com/order/catalog/product/22400089productDetailPagePosokhov, Y. O., Kyrychenko, A. (2013). Effect of acetone accumulation on structure and dynamics of lipid membranes studied by molecular dynamics simulations. computational Biology and Chemistry, 46, 23-31.Rodal, S. K., Skretting, G., Garred, O., Vilhardt, F., Deurs, B. V., Sandvig, K. (1999). Extraction of Cholesterol with Methyl-beta -Cyclodextrin Perturbs Formation of Clathrin-coated Endocytic Vesicles. Molecular Biology of the Cell, 10(4), 961-974.Sigma. (2003). Tween-20 (P5927) Product Information. SigmaAldrich. Retrieved October 6, 2014, from https//www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Produc t_Information_Sheet/1/p5927pis.pdfStrober, W. (2011). Trypan blue exclusion test of cell viability. occurrent Protocols in Immunology, 21(3B), A.3B.1A.3B.2.Tsunoda, T., Imura, T., Kadota, M., Yamazaki, T., Yamauchi, H., Kwon, K. O., et al. (2001). Effects of lysozyme and bovine serum albumin on membrane characteristics of dipalmitoylphosphatidylglycerol liposomes. Colloids and Surfaces B Biointerfaces, 20(2), 155-163.USMA. (2012). Metric System Temperature. U.S. Metric Association. Retrieved October 6, 2014, from http//lamar.colostate.edu/hillger/temps.htmVentosa, A., Nieto, J., Oren, A. (1998). Biology of Moderately Halophilic Aerobic Bacteria. Microbiology and Molecular Biology Reviews, 62(2), 504-544.Zhao, Y., Zhang, W., Kho, Y., Zhao, Y. (2004). Proteomic Analysis of Integral Plasma Membrane Proteins. Analytical Chemistry, 76(7), 1817-1823.

Research Strategy of Service Quality Assessment

Research Strategy of Service Quality AssessmentMethodologyFor this mull survey, strategy impart be employ in effectuate to collect data. Survey researchers are quite distinct from the general surveys conducted for different purposes, including merchandising survey. According to Tanur(1982) surveys are slackly meant of collecting data related to characteristics, actions, or opinions of a large group of people, referred to as a population. The surveys conducted for research purposes are focused on advancement of knowledge within a specific domain. Research survey data give the sack be lay in by different methods, including interviews, headingnaires and observations. In terms of questionnaires, the data sess be collected either through open-ended questions in which participants need to yield details answer or closed questions in which participants need to provide select single or multiple answers from the choice of answers. According to Groves et al. (2013) survey data will be biased if the deal of the survey is not properly followed, which includes roleing, writing, collecting and analysing sub-processes. Figure 1 presents the typical data assembling process as describe by Groves et al.(2013) Figure 1 A survey from process prospective (Groves et. al. 2013,pp.149)For the research survey, strategy will be used and data will be collected through set of questionnaires which respondent will be able to complete themselves. The reason for using asker as data collection method was because, firstly, this research is using survey strategy and harmonise to Saunders et al. (2012) questioner is one of the to the highest degree widely used data collection methods within survey strategy. Secondly, since this research will be providing the closed answers which will be used for quantitative data compend, therefore, questionnaire data collection method is more suited for this research.In terms of try out, since its pilot data collection and research is focused on general consumer acquaintance approximately the service delivery and expected quality evaluated through the prism of culture, therefore, as describe by Saunders et al. (2012) probability based sample distribution technique will be used to identify a group from population for data collection. For quality of data, research has applied stratified random sampling by using the one attribute that has the basic level of experience in terms of service delivery from the population. The validity or according to Saunders et al. (2012) core validity, questions in a survey provide adequate representation for research questions. As describe by Saunders et al. (2012) validity of the content can be evaluated through literature review, which has been provided for this research. For the reliability and consistency in response to the questionnaire, the research is confined to only closed questions with multiple-choice single selection answers, and questions are all design using simple language. The questions on the survey are design based upon the research questions, and all the questions in the survey are closed question that is multiple-choice single selection in order to collect consistent data. The order and flow of the questions are carefully design to avoid confusion and misinterpretation. Similarly, in order to avoid blank or missed questions the questions are presented on the same page, Please see the Appendix for actual questionnaire in the survey. Although the research is evaluating the research question using qualitative research methodology, but the data collected through a survey is processed by quantitative data analysis. The data collected through a survey is considered to numerical data, and exploratory data analysis(EDA) approach will be used to process it. According to Saunders et al. (2012) EDA approach for data analysis emphasis on using diagrams and tables for understanding and exploring data. Since this is pilot research, therefore, as describe by Sa unders et al. (2012) EDA approach is useful for initially stages of the research.Research instrument and data collectionThe survey is divided into two sections first section contains demographic questions two get more understandings about the respondent and their classification. The second section contains the statement about the service encounter which participants had to rate using 7-points Likert scale starting from 1 (strongly disagree) to 7 (strongly agree). Before attempting to rate the statements participants were asked to think of the service they have received from the industries exchangeable banks, retail, etc. and use the experience to rate the statements. The survey is conducted electronically by creating the questionnaire on a free online survey conducting website which provides functionality to share a fall in to the survey. The link to the survey is shared through email and also via different electronic forums, The data collected through the survey is analysed and evaluated after downloading it from the website. Sample data comprised of two sources that is the student of universities and colleges. A total of hundred responses was received out of several hundred survey requests. As the research aims to analyse the effect of culture on customer perception of service, therefore, the sampling framework was clustered into two major group based on their ethnicity that is white and non-white. Population targeted for this research was local consumers supporting in UK and had received service from industries like banks, retail, etc. in last few months. Participants from the sample are chosen using researchers own intuition and chosen with purpose. (Tellstrom et al., 2006). In order to further evaluate participant demographic information like age and education has also been collected.MeasurementIn order to posting a perception of service quality and evolution process, in this SERVQUAL scale will be used, which was developed by Parasuraman et al. (19 91). SERQUAL provides 22-items scale, spanning five dimensions (tangibles, reliability, responsiveness, assurance, and empathy), of service quality expectations and, which has been evaluated and validate in numerous studies (Donthu and Yoo, 1998). Although researchers have identified some shortcoming in using SERVQUAL, but the model is most widely referenced and practiced among unhomogeneous industries. The questionnaires in this study were design to capture the customer perceptions of service quality based on five quality dimension of SERVQUAL. For gauging the culture of the survey participant, the study will use the Hofstedes framework of cultural dimensions. Several scholars have proposed alternative frameworks to conceptualize and operational culture to overcome the shortcoming of Hofstedes framework. However, Hofstedes framework is still the most widely used cultural framework in different fields of study, including psychology, sociology, marketing, or management (Soares et al ., 2007). The research has used the approach of grouping the participant according to their individual scores on Hofstedes cultural dimension scale resulting in better understanding the role of culture in shaping service perception (Guesalaga et al ., 2016). The survey has been conducted within UK, and participants predominantly belong to western culture but some of the participant involved has a different culture which can give better understand of cultural effect on service quality perception.ReferencesGuesalaga, R. Pierce, M. and Scaraboto, D. (2016), Cultural influences on expectations and evaluations of service quality in emerging markets, internationalistic Marketing Review, 33(1), pp. 88-111Parasuraman, A. Berry, L. and Zeithaml, V.A. (1991), Refinement and reassessment of the SERVQUAL scale, Journal of Retailing, .67(4), pp. 420-450.Saunders, M., Lewis, P. and Thornhill, A. (2012) Research methods for business students. 6th edn. Harlow Pearson.Tanur, J.M.(1982) Advances in methods for large-scale surveys and experiments, in R. Mcadams, N.J. Smelser, D.J. Treiman (eds.), Behavioral and Social Science Research A National Resource, Part II. Washington, D.C. National Academy Press.Groves,R. M., Fowler, F.J., Couper, M.P. Jr., Lepkowski, J. M., Singer, E. and Tourangeau, R. (2013), Survey Methodology, First Edition,John Wiley SonsDonthu, N. and Yoo, B. (1998),Cultural Influences on Service Quality Expectations, Journal of Service Research,1(2), pp.178-186Soares, A. M. Farhangmehr, M. and Shoham, A. (2007), Hofstedes dimensions of culture in international marketing studies, Journal of Business Research, 60, pp. 277 284

Schizophrenia :: science

This mental disorder. However, it is proven that one is more apt to obtain schizophrenia if there is a fragment in their family that has had this disease already leaving those who do not have a family member with schizophrenia to be very unlikely to acquire it. other way of acquiring schizophrenia in the inherited sense is through a dietary factor. This would mean that a certain food would trigger schizophrenia in a household present in the member of the family that eats that food (Torrey 80). Early in this disease, there may be obsession with religion, matters of the supernatural, or abstract causes of creation. Speech may be characterized by unclear symbolisms. Later, words and phrases may become puzzling, and these can only be understood as part of the persons private fantasy world. People who have been ill with schizophrenia for a long time often have oral communication patterns that are disoriented and aimless and deficient of meaning to the casual observer. Sexual activity is frequently altered in mental disorders. Homosexual concerns may be associated with all psychoses but are most prominent with paranoia. Doubts concerning sexual identity, exaggerated sexual needs, altered sexual performance and fears of intimacy are prominent in schizophrenia. The process of regression in schizophrenia is accompanied by increased self-fixation, isolation, and masturbatory behavior. The schizophrenic person finds himself or herself in a painful dilemma. He or she retreats from personal intimacy or closeness because of the intense fear that closeness will be followed by rejection or harm. This retreat from intimacy leaves the person solitary and isolated. This dilemma often becomes the care-takers dilemma. The care-taker wishes to form a productive emotional bond but at the same time seeks to lessen the persons anxiety. The schizophrenic person, who moves toward emotional closeness, will eventually increase anxiety. The dopamine theory of schizophrenia is based on the action of the neuroleptic drugs, better known as antipsychotic drugs. Neuroleptics are the drugs of choice for treating the symptoms of schizophrenia. The neuroleptics are believed to block the dopamine receptors in the brain, limiting the activity of dopamine and reducing the symptoms of schizophrenia. Amphetamines, just the opposite, enhance dopamine transmission. Amphetamines rise an excess of dopamine in the brain and can provoke the symptoms of schizophrenia in a schizophrenic client. In large doses, amphetamines can simulate symptoms of paranoid schizophrenia in a nonschizophrenic person. Some symptoms of schizophrenia are due basically to hyperdopaminergic activity.

Brave New World - Is Individuality a Threat to Society, or a Gift to So

As man has progressed through the ages, there has been, essentially, whizz purpose. That purpose is to arrive at a utopian society, where everyone is happy, infirmity is nonexistent, and strife, anger, or sadness is unheard of. Only happiness exists. But when confronted with Aldous Huxleys gay New populace, we come to realize that this is not, in fact, what the human soul really craves. In fact, Utopian societies are much worse than those of today. In a utopian society, the individual, who among others composes the society, is lost in the melting pot of semblance and world of uninterest. The theme of Huxleys Brave New World is community, identity, and stability. Each of these three themes represents what a Brave New World society needs to have in order to survive. According to the hot world controllers, community is a result of identity and stability, identity is a part of genetic engineering, and stability is what everyone desires to achieve. These themes are represented i n the book by the symbolic meaning of the phrase Children are from bottles and the hypnotic phrase Everybody belongs to everybody else. For a better understanding it is useful to explore these themes in detail. Community refers to the thought of one whole unit. Everyone is connected, by their actions toward each other in every day life, sexual desires, and what they do to remove the feeling of horrible emotions. This connectedness and lie, and its effects empennage be seen in the character of Bernard, a person who hates what society has fuck off.The year is A.F. 632 (After Ford Ford is the equivalent to God in Brave New World and with the available technology, citizens are mass produced. But have an underlying theme in common. The stability of this brave new w... ...ch. It is this willingness of man to make the same fracture twice that in 2000 the ideas in Brave New World do not seem that far off base. Most people thought that with the develop of the Soviet Union it would put a n end to the suffering and an all-controlling government. But with an influx of clones, test tube babies, government controls of television, needless violence, and the search for the perfect mood alter drug. Who is to say that Brave New World is not earth in fifty years? As more people lose their individuality they become connected with community. It is with this connection that they begin to let others control their lives and humanity is already headed in that direction. Brave New World should not only be seen as a great piece of science fiction. It should be seen as a warning. Of what can happen when people live up to the influence of outside sources.

Sir Gawain and the Green Knight Essay -- essays research papers fc

Women, Courtly Love and the Creation Myth in Sir Gawain and the Green Knight     Sir Gawain and the Green Knight, a great epic written in fourteenth century Europe by the Pearl poet, emphasizes the opposition of Christian hunch to Courtly love in the 13th century through the plight of Sir Gawain, one of the great knights of the Arthurian round table. By examining the women in the poem, Gawains dilemma becomes a metaphor for the contrast of these two distinct types of love. The poem looks upon the Virgin bloody shame as the representative of spiritual love, obedience, chastity, and life (Warner 9). In contrast, Morgan le Fay and Bertilaks wife appear to be representing courtly love, disobedience, lust and death. This conflict between courtly love and spiritual love demonstrates the drastically weakened religious values behind chivalry. An interesting parallel to Sir Gawain and the Green Knight is the story of original sin in the Garden of Eden. Gawains temptatio n correlates to the temptation of Adam, which is rooted in the sins of the flesh. The women in the story seem to accentuate the down inclination of Gawain, which mirrors the downfall of Arthurs court, as well as mans fall from grace in the garden.      Originally, the first duty of a knight was to be at the service of his church. However, with the rise of courtly love, knights began to give their devotion to their mistress alternatively than God. This elevated the churchs mistrust of women and the flesh. The characterization of Bertilaks wife is not unlike that of Eve, a temptress who would bring both happiness and despair to her man. One interesting structure to this story is that, like courtly love, possession of power seems to be shifted into the hands of the women. The wife of Bertilak operates unassisted against Gawain in the bedroom as the hunter and the aggressor. The great fair(prenominal) power in the story, however, comes from Morgan le Fay, the evil stepsister of Arthur. She is strong enough to move into Bertilaks castle, turn him green and order him to walk and talk with a separate head.      The Virgin Mary also plays a prominent role in the tale. It seems as if Mary and Gawain have a relationship based on a special untainted Christian love. That Gawain is Marys knight is made clear in the scene where he is robed for battle. That all h... ...t. The women in this tale serve as a medium for comparing the spiritual journey of Sir Gawain to the creation myth. With Mary as a symbol of perfection and Lady Bertilak as an Eve-like symbol of temptation, the feature that he is torn between them displays his humanness just as Adam was torn between the word of God and the love of Eve. In this case, Morgan le Fay would plausibly be considered the serpent in the garden, the instigator of the conflict in the story. Because of the story of Eve, women were frequently looked upon in medieval times as cunning, untrustwo rthy and generally evil. Women in the story of Sir Gawain and the Green Knight are looked upon with the male concept that flesh is evil and will lead to mans downfall, which is highlighted by the contrast of Courtly love to Christian love. Works CitedAbrams, M.H. The Norton Anthology of English Literature. New York W.W. Norton & Company, Inc., 1993. De Roo, Harvey. "Undressing Lady Bertilak Guilt and Denial in Sir Gawain and theGreen Knight." The Chaucer Review 27 (1993) 305-24.Warner, Marina. Alone of all Her Sex The Myth and the craze of the Virgin Mary. New York Alfred A. Knopf, Inc., 1976.